Apoptosis: Methods and Protocols, Second Edition by Sharad Kumar, Loretta Dorstyn (auth.), Peter Erhardt, Ambrus

By Sharad Kumar, Loretta Dorstyn (auth.), Peter Erhardt, Ambrus Toth (eds.)

The skill to realize and quantify apoptosis, to appreciate its biochemistry, and to spot its regulatory genes and proteins is important to biomedical examine. In Apoptosis: tools and Protocols, moment Edition, professional researchers describe the innovations to most sensible examine the severe steps all in favour of the apoptotic technique. provided from a number of varied study views, the quantity comprises sections protecting detection of apoptosis, detection of non-apoptotic mobilephone demise, transformations of apoptotic proteins in the course of apoptosis, the research of its significant regulators, in addition to research of apoptosis in several organs and in version organisms. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, easily reproducible protocols, and notes on troubleshooting and averting identified pitfalls.

Comprehensive and state of the art, Apoptosis: equipment and Protocols, moment Edition constitutes a key technical connection with the numerous methodologies utilized in the sphere, and gives rookies and skilled researchers robust instruments to light up the phenomena of programmed mobile death.

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31. 32. 33. 34. 35. 36. 17 ubiquitin-protein ligase Nedd4 during apoptosis. J. Biol. Chem. 273, 13524–13530. Kaufmann, S. , Davidson, N. , and Poirier, G. G. (1993). Specific proteolytic cleavage of poly(ADPribose) polymerase: an early marker of chemotherapy-induced apoptosis. Cancer Res. 53, 3976–3985. Lazebnik, Y. , Kaufmann, S. , Poirier, G. , and Earnshaw, W. C. (1994). Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371, 346–347. , Lees-Miller, S. , Chan, D.

Specific proteolytic cleavage of poly(ADPribose) polymerase: an early marker of chemotherapy-induced apoptosis. Cancer Res. 53, 3976–3985. Lazebnik, Y. , Kaufmann, S. , Poirier, G. , and Earnshaw, W. C. (1994). Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371, 346–347. , Lees-Miller, S. , Chan, D. , Smith, G. , et al. (1996). DNA-dependent protein kinase catalytic subunit: a target for an ICE-like protease in apoptosis. EMBO J. 15, 3238–3246. , and Nagata, S.

And Darzynkiewicz, Z. (2002). Kinetics of HL-60 cell entry to apoptosis during treatment with TNF-a or camptothecin assayed by stathmo-apoptosis method. Cytometry 47, 143–9. 15. T. J. (1994). Annexin V for flow cytometric detection of phosphatidylserine expression of B cells undergoing apoptosis. Blood 84, 1415–20. 16. , Schutte, B. M. (1998). Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry. 31, 1–9. 17. , Grignani, F. and Riccardi, C.

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